Gelatin methacryloyl (GelMA) is a double-bond modified gelatin, which can be crosslinked to form hydrogels by ultraviolet and visible light under the condition of adding photoinitiators.GelMA hydrogels have excellent biocompatibility and adjustable mechanical properties, and fluorescent GelMA is chemically grafted fluorescent molecules onto GelMA molecules, which can be made to have specific fluorescent colors by changing the types of fluorescent molecules. This chemical labeling method avoids the drawbacks of physical mixing or electrostatic adsorption where the fluorescent molecules tend to diffuse out of the system, and also avoids the disadvantage of uneven imaging of fluorescent particles, which is widely used in biomedical fields such as tissue engineering, 3D printing, and drug delivery.

Molecular Structure:

Product Type:

Steps for use:

1. Preparation of 0.5mg/mL initiator standard solution

Add 20mL of solvent (DMSO, sterilized water, PBS, etc.) to the brown glass bottle containing the initiator LAP, and dissolve for 30min in a 37 ℃ water bath with heat, shaking several times to fully dissolve.

2. Prepare fluorescent GelMA solution (recommended concentration 5-20%)

Take appropriate amount of fluorescent GelMA into a centrifugal tube, add the prepared LAP standard solution, dissolve at 50 ℃ water bath for 30min, shaking several times, fully dissolved.

Curing into gel:

1. Use 405nm blue light to irradiate the above solution containing initiator, about 20~30s can be cured into glue (irradiation time is related to the concentration of fluorescent GelMA and the blue light source, according to the required strength of the glue can be adjusted to the appropriate curing time);

2. if use 2959 initiator, need to use 365nm UV light for irradiation and appropriate extension of the irradiation time (LAP is more efficient than 2959 initiator, shorter time), UV will hurt the skin and eyes, pay attention to protection.

2D cell culture

1. Dissolve the LAP-containing fluorescent GelMA solution according to the above dissolution protocol and pass it through 0.22μm filter membrane while it is still hot;

2. Add appropriate amount of fluorescent GelMA solution into the well plate to cover the bottom surface of the plate;

3. UV light curing for about 30-60s. 4;

4. Wash the cured fluorescent GelMA with PBS several times, or use culture medium for rinsing;

5. After cell counting, inoculate the cell suspension into the well plate, observe the wall attachment after 24 h and carry out the subsequent cell culture.

Precautions

1. It is recommended to increase the irradiation time appropriately to obtain better curing effect. Recommended time: fluorescent GelMA concentration ≤15%, irradiation time ~30s; fluorescent GelMA concentration >15%, irradiation time ~20s;

2. Due to the temperature-sensitive nature of fluorescent GelMA, its solution is easy to be cured under low temperature. Therefore, please do not repeatedly freeze and thaw for many times when using, so as not to affect the performance of the product;

3. Using 2959 initiator requires higher temperature to dissolve in water or PBS (solubility in water ≥ 5mg/mL);

4. LAP initiator solution can be stored for 12 months at 4℃ under light protection, but it is recommended to use it now;

5. It is recommended to use LAP initiator for cellular experiments because blue light irradiation is gentler to cells than UV.

6. After adding LAP, the solution needs to be protected from light and the centrifuge tube can be wrapped with tin foil.

Production date see package

Suzhou Xianjue New Material Technology Co.

Tel：18068408145

Address: No.1360, Jinji Lake Avenue, Suzhou, China.