Collagen methacryloyl (COMA) is a double-bond modified collagen that can be crosslinked to form hydrogels by ultraviolet and visible light with the addition of photoinitiators.COMA hydrogels have excellent biocompatibility and adjustable mechanical properties, and are widely used in biomedical fields such as tissue engineering, 3D printing, and drug delivery.

Molecular Structure:

Steps for use:

1. Preparation of 0.5mg/mL initiator standard solution

Add 20mL of solvent (sterilized water, PBS, etc.) to the brown glass bottle containing the initiator LAP, and dissolve for 30min at room temperature, shaking several times to fully dissolve.

2. Prepare COMA solution (recommended concentration is 10-20%, minimum 7.5% can be gelatinized)

Take an appropriate amount of COMA into a centrifuge tube, add the prepared LAP standard solution, dissolve for 30 min at 37 ℃ in a water bath, oscillate several times and fully dissolve.

2D cell culture:

1. Dissolve the LAP-containing COMA solution according to the above dissolution protocol and pass it through 0.22μm filter membrane while it is still hot;

2. Add appropriate amount of COMA solution into the well plate to cover the bottom of the plate. 3;

3. UV light curing for about 30-90s (irradiation time is related to COMA concentration and blue light source);

4. Wash the cured COMA with PBS several times, or use culture medium for rinsing;

5. After cell counting, inoculate the cell suspension into the well plate, and observe the affixation situation and subsequent cell culture after 24h.

Precautions:

1. It is recommended to increase the irradiation time appropriately to obtain better curing effect. Recommended time: 30 ~ 90s;

2. It is recommended to use the product now, please do not repeatedly freeze and thaw, so as not to affect the performance of the product;

3. Use 2959 initiator needs higher temperature to dissolve in water or PBS (solubility in water ≥ 5mg/mL);

4. LAP initiator solution can be stored for 12 months at 4℃ under the protection of light, but it is recommended to use it now;

5. It is recommended to use LAP initiator for cell experiments because blue light irradiation is gentler to cells than UV;

6. The company is only responsible for the quality control of the material itself, but not for the subsequent additional biological experiments and other results unrelated to the quality control of the material itself.

The production date is shown in the package:

Suzhou Xianjue New Material Technology Co.

Tel: 18068408145

Address: No. 1360, Jinji Lake Avenue, Suzhou, Yue Long Business, 2nd Floor.