Hyaluronic acid methacryloyl (HAMA) is a double-bond modified hyaluronic acid that can be crosslinked to form a hydrogel by UV and visible light with the addition of photoinitiators.HAMA hydrogels have excellent biocompatibility and adjustable mechanical properties, and are widely used in biomedical fields such as tissue engineering, 3D printing, and drug delivery.

Molecular Structure:

Steps for use:

1. Preparation of 0.5mg/mL initiator standard solution

Add 25mL of solvent (sterilized water, sterile PBS, etc.) to the brown glass vial containing the initiator LAP, and dissolve for 30min at room temperature, shaking several times to fully dissolve.

2. Prepare HAMA solution (recommended concentration 2-10%)

Take an appropriate amount of HAMA into a centrifuge tube, add the prepared LAP standard solution, dissolve for 30min at room temperature, oscillate several times and fully dissolve.

Curing into gel:

1. Use 405nm blue light to irradiate the above solution containing initiator, about more than 30s can be cured into glue (irradiation time is related to the concentration of HAMA and the blue light source, according to the required strength of the glue can be adjusted to the curing time);

2. if use 2959 initiator, need to use 365nm UV irradiation and extend the irradiation time (LAP is more efficient than 2959 initiator, shorter time), UV will hurt skin and eyes, pay attention to protect.

Precautions:

1. It is recommended to increase the irradiation time to get better curing effect. Recommended time: HAMA concentration ≤ 5%, irradiation time ~ 30s; HAMA concentration > 5%, irradiation time ~ 20s. 2;

2. Use 2959 initiator needs higher temperature to dissolve in water or PBS (solubility in water ≥5mg/mL);

3. LAP initiator solution can be stored for 12 months at 4 ℃ and protected from light, but it is recommended to use it now;

4. It is recommended to use LAP initiator for cellular experiments because blue light irradiation is gentler to cells than UV;

5. After adding LAP, the solution needs to be protected from light and the centrifuge tube can be wrapped with tin foil;

6. Our company is only responsible for the quality control of the material itself, but not for the results of subsequent biological experiments or other results not related to the quality control of the material itself.

The date of production is shown in the package.

Suzhou Xianjue New Material Technology Co.

Tel: 18068408145

Address: 2nd Floor, No.1360 Jinji Lake Avenue, Suzhou, China.