Dextran methacryloyl (DexMA) is a double-bonded modified dextran that can be crosslinked to form hydrogels by UV and visible light with the addition of photoinitiators.DexMA hydrogels have excellent biocompatibility and tunable mechanical properties, and are widely used in biomedical fields such as tissue engineering, 3D printing, and drug delivery.

Molecular Structure:

Steps for use:

1. Preparation of 0.5mg/mL initiator standard solution

Add 20mL of solvent (sterilized water, PBS, etc.) to the brown glass bottle containing the initiator LAP, and dissolve for 30min in a 37 ℃ water bath with heat, shaking several times to fully dissolve.

2. Preparation of DexMA solution (recommended concentration 5-15%)

Take appropriate amount of DexMA into a centrifuge tube, add the prepared LAP standard solution, dissolve at room temperature for 30min, shaking several times, fully dissolved.

Curing into gel:

1. Use 405nm blue light to irradiate the above solution containing initiator, about 20 ~ 30s can be cured into adhesive (irradiation time is related to the concentration of DexMA and the blue light source, according to the strength of the required adhesive can be adjusted to the curing time);

2. if use 2959 initiator, need to use 365 nm UV light for irradiation and extend the irradiation time (LAP is more efficient than 2959 initiator, shorter time), UV will hurt the skin and eyes, pay attention to protect.

Precautions:

1. It is recommended to increase the irradiation time to get better curing effect. Recommended time: DexMA concentration ≤ 10%, irradiation time ~ 30s; HAMA concentration > 10%, irradiation time ~ 20s. 2;

2. Use 2959 initiator needs higher temperature to dissolve in water or PBS (solubility in water ≥ 5 mg/mL);

3. LAP initiator solution can be stored at 4 ℃ under light protection for 12 months, but it is recommended to use it now;

4. It is recommended to use LAP initiator for cellular experiments because blue light irradiation is gentler to cells than UV;

5. After adding LAP, the solution needs to be protected from light and the centrifuge tube can be wrapped with tin foil;

6. We are only responsible for the quality control of the material itself, but not for the results of the subsequent additional biological experiments and other results not related to the quality control of the material itself.

The date of production is shown in the package:

Suzhou Xianjue New Material Technology Co.

Tel: 18068408145

Address: No. 1360, Jinji Lake Avenue, Suzhou, Yue Long Business, 2nd Floor.