Chondroitin sulfate methacryloyl (ChSMA) is a double bond modified chondroitin sulfate that can be crosslinked to form a hydrogel by UV and visible light with the addition of photoinitiators.ChSMA hydrogels have excellent biocompatibility and adjustable mechanical properties, and are widely used in biomedical fields such as tissue engineering, 3D printing, and drug delivery.

Molecular Structure:

Steps for use:

1. Preparation of 0.5 mg/mL initiator standard solution

Add 20mL of solvent (sterilized water, PBS, etc.) to the brown glass bottle containing the initiator LAP, and dissolve for 30min in a 37 ℃ water bath with heat, shaking several times to fully dissolve.

2. Prepare ChSlMA solution (recommended concentration 4-10%)

Take appropriate amount of ChSMA into a centrifuge tube, add the prepared LAP standard solution, dissolve for 30 min at room temperature, oscillate several times and fully dissolve.

Curing into gel:

1. Use 405nm blue light to irradiate the above solution containing initiator, about 30s can be cured into glue (irradiation time is related to the concentration of ChSMA and the blue light source, according to the required strength of the glue can be appropriately adjusted curing time);

2. if use 2959 initiator, need to use 365 nm UV irradiation and extend the irradiation time (LAP is more efficient than 2959 initiator, time is shorter), UV light will hurt the skin and eyes pay attention to protect.

Precautions:

1. It is recommended to increase the irradiation time to get better curing effect. Recommended time: ChSMA concentration ≤5%, irradiation time ~ 40 s; ChSMA concentration >5%, irradiation time ~ 30 s. 2;

2. Use 2959 initiator needs higher temperature to dissolve in water or PBS (solubility in water ≥ 5 mg/mL);

3. LAP initiator solution can be stored for 12 months at 4℃ protected from light, but it is recommended to use it now;

4. It is recommended to use LAP initiator for cellular experiments, blue light irradiation is gentler to cells than UV;

5. After adding LAP, the solution needs to be protected from light, and the centrifuge tube can be wrapped with tin foil.

6. Our company is only responsible for the quality control of the material itself, but not for the subsequent additional biological experiments and other results unrelated to the quality control of the material itself.

See the package for production date.

Suzhou Xianjue New Material Technology Co.

Tel: 18068408145

Address: No.1360, Jinji Lake Avenue, Suzhou, China.