Silk fibroin methacryloyl (SilMA) is a double-bonded modified silk protein that can be crosslinked to form hydrogels by UV and visible light with the addition of photoinitiators.SilMA hydrogels have excellent biocompatibility and tunable mechanical properties, and are widely used in biomedical fields such as tissue engineering, 3D printing, and drug delivery.

Molecular Structure:

Steps for use:

1. Preparation of 0.5 mg/mL initiator standard solution

Add 20 mL of solvent (DMSO, sterilized water, PBS, etc.) to the brown glass bottle containing the initiator LAP, and dissolve for 30 min in a 37 ℃ water bath with heat, shaking several times to fully dissolve.

2. Prepare SilMA solution (recommended concentration 10-20%)

Take an appropriate amount of SilMA into a centrifuge tube, add the prepared LAP standard solution, dissolve for 30 min at room temperature, shaking several times to fully dissolve. (Avoid violent ultrasonication, high temperature and strong shear, etc.)

Curing into glue:

1. Use 405nm blue light to irradiate the above solution containing initiator, about 30s can be cured into adhesive (irradiation time is related to the concentration of SilMA and blue light source, according to the strength of the adhesive can be adjusted to the appropriate curing time);

2. If using 2959 initiator, you need to use 365nm UV light to irradiate and extend the irradiation time appropriately (LAP is more efficient than 2959 initiator, shorter time), UV light will hurt the skin and eyes attention to protection.

Precautions:

1. It is recommended to increase the irradiation time to get better curing effect. Recommended time: 30 s. 2;

2. Use 2959 initiator requires higher temperature to dissolve in water or PBS (solubility in water ≥ 5mg/mL);

3. LAP initiator solution can be stored for 12 months at 4℃ protected from light, but it is recommended to use it as it is prepared;

4. It is recommended to use LAP initiator for cell experiments, blue light irradiation is gentler to cells than UV;

5. The silk protein solution is a semi-stable sol, easily induced by external stimuli such as strong shear, ultrasound, high temperature, organic solvents and other molecular self-assembly, precipitation or gelation behavior, it is recommended to use now.

6. It is normal that there is a little insoluble substance in the silk protein solution, it is recommended to remove the insoluble substance by centrifugation or filtration to avoid inducing more silk protein precipitation;

7. Our company is only responsible for the quality control of the material itself, but not for the subsequent additional biological experiments and other results unrelated to the quality control of the material itself.

See the package for production date.

Suzhou Xianjue New Material Technology Co.

Tel: 18068408145

Address: No. 1360 Jinji Lake Avenue, Suzhou, Yue Long Business, 2nd Floor.