Polyether F127 diacrylate (F127DA) is a double-bond modified polyethylene glycol-polypropylene glycol-polyethylene glycol triblock copolymer, which can be rapidly crosslinked and cured into a gel under the action of photoinitiators in both UV and visible light.F127DA also possesses good temperature-sensitive gelation (thermo-gelation) and biosafety, and has been widely used in a variety of biomedical fields, such as drug carrier, wound dressing, cellular microcarrier, bio-3D printing, etc. dressings, cell microcarriers, and bio-3D printing.

Molecular structure:

Steps for use:

1. Prepare 0.5 mg/mL initiator standard solution

Add 20 mL of solvent (sterilized water, PBS, etc.) to the brown glass vial containing the initiator LAP, and dissolve for 30 min at room temperature, shaking several times to fully dissolve.

2. Preparation of F127DA solution (recommended concentration 5-30%)

Take appropriate amount of F127DA into a centrifuge tube, add the prepared LAP standard solution, dissolve at 2-8 ℃ for 30 min, shaking several times, fully dissolved (concentration <20% can be dissolved by stirring at room temperature).

Curing into gel:

1. Use 405 nm blue light to irradiate the above solution containing the initiator, about 10-30 s can be cured into glue (irradiation time is related to the concentration of F127DA and the blue light source, according to the required strength of the glue can be adjusted to the curing time);

2. if use 2959 initiator, need to use 365 nm UV light for irradiation and extend the irradiation time (LAP is more efficient than 2959 initiator, time is shorter), UV light will hurt the skin and eyes pay attention to protect.

Precautions:

1. It is recommended to increase the irradiation time to get better curing effect. Recommended time: F127DA concentration ≤10%, irradiation time ~ 20 s; F127DA concentration >10%, irradiation time ~ 30 s. 2;

2. The use of 2959 initiator requires higher temperature to dissolve in water or PBS (solubility in water ≥ 5 mg/mL);

3. LAP initiator solution can be stored for 12 months at 4°C protected from light, but it is recommended to use it now;

4. After adding LAP, the solution needs to be protected from light and the centrifuge tube can be wrapped with tin foil;

5. It is recommended to use LAP initiator for cellular experiments, blue light irradiation is gentler to cells than UV;

6. F127DA solution has thermogenic gel properties, in the preparation of the solution, it is recommended that the solution is dissolved at 2-8 ℃, during which it is shaken several times;

7, the concentration of ≥ 20% of the F127DA solution at room temperature will appear gelation phenomenon, the higher the concentration, the more obvious gelation phenomenon, the process is reversible, the temperature is reduced to 2 ~ 8 ℃ will be restored to the solution state;

8. Our company is only responsible for the quality control of the material itself, and is not responsible for the results of subsequent additional biological experiments and other results unrelated to the quality control of the material itself.

The date of production is shown in the package:

Suzhou Xianjue New Material Technology Co.

Tel: 18068408145

Address: No. 1360, Jinji Lake Avenue, Suzhou, Yue Long Business, 2nd Floor.